IGVFFI3544BRUD

released

validated

File Set

IGVFDS8794YVNF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep2_S79

md5sum

2340140055a78e0521b9bace6e27762f

Content MD5sum

e697b638f28d06a6f6fb331b0161224d

File Size

25 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinF-PCR4-Rep2_S79_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TACTTAGC:TCTC

Average Read Length

Read Count

1269426

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5744WHTT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library