IGVFFI3522NTQE

released

validated

File Set

IGVFDS7919SMDO(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF1-BinC-PCR3-Rep1_S165

md5sum

dd8f78a82c4839fbc160a062ba75dab1

Content MD5sum

666e801ce3e01ffe960d3262ff9bc500

File Size

34 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF1-BinC-PCR3-Rep1_S165_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCGGAATG:ATTG

Average Read Length

Read Count

1562454

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3123SZYY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library