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IGVFFI3515IWOR

released
validated
File Set
IGVFDS9674AATW(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
R1 reads from sequencing run 3
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispri-gex-rep3-rep3-fastq-lane2-R1
md5sum
c6ab1897b31e089e3c4695e20e41e0c2
Content MD5sum
aeece9731966d129c95a6737946e659d
File Size
5.7 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/GEX_CRISPRi_D3_S3_L002_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
3
Lane
2
Sequencing Kit
NovaSeq 6000 S4 Reagent Kit v1.5
Illumina Read Type
R1
Average Read Length
26
Read Count
278051968

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
335 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI8510AXNZ, IGVFFI3515IWOR
Charles Gersbach, Duke
982 B
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke