Home

IGVFFI3479RWOJ

released
validated
File Set
IGVFDS6229XAIE(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-BinD-PCRrep5_R1
md5sum
b64a6570f3a8bc03403b8f5b43fc68eb
Content MD5sum
11eb6a6a5df949aa769b8ab0e067b768
File Size
3.1 MB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep2-FFrep3-BinD-PCRrep5_S239_R1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R1
Index
TGCAAGTA:ACTG
Average Read Length
150
Read Count
98689

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
hg19 variant effects
Jesse Engreitz, Stanford
6.7 KB
validated