IGVFFI3467MIOR

released

validated

File Set

IGVFDS8557UXEJ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_FAM3C_Auxin0hrs-FF2-BinA-PCR4-Rep1_S181

md5sum

122dc2ae8b77b26aac39fbe7ffcb7edc

Content MD5sum

13c9eafb6a057431cb9ba2833170b475

File Size

46 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/FAM3C-Auxin0hrs-FF2-BinA-PCR4-Rep1_S181_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCGCCTT:ATTG

Average Read Length

Read Count

2113034

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1718NCDJ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of FAM3C) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library