IGVFFI3454BUML

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI3454BUML/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI3454BUML",
    "aliases": [
        "jesse-engreitz:auxiliary_files_CRUDO_TAPseq-Auxin6hrs-Rep2_S2_R1"
    ],
    "assay_titles": [
        "TAP-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011972/",
        "component": "functional characterization"
    },
    "content_md5sum": "32c48c5cb94be147f14a0e04505c25af",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-06-03T19:17:10.048409+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS4057PFBU/",
        "accession": "IGVFDS4057PFBU",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM8511JOMW/",
                "accession": "IGVFSM8511JOMW",
                "classifications": [
                    "cell line"
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0002824/",
                        "term_name": "HCT116"
                    }
                ],
                "summary": "HCT116 cell line, male, Homo sapiens treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "summary": "gRNA sequencing for mixed TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci"
    },
    "file_size": 72826284,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI3454BUML/@@download/IGVFFI3454BUML.fastq.gz",
    "illumina_read_type": "R1",
    "input_file_for": [
        "/tabular-files/IGVFFI1012PVIG/",
        "/tabular-files/IGVFFI4629HCQH/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/jesse-engreitz/",
        "title": "Jesse Engreitz, Stanford"
    },
    "lane": 2,
    "loci_list_for": [],
    "maximum_read_length": 28,
    "md5sum": "0e3be518c629ad778ce748f65c05223b",
    "mean_read_length": 28,
    "minimum_read_length": 28,
    "read_count": 2955304,
    "release_timestamp": "2024-07-10T20:26:55.002015+00:00",
    "s3_uri": "s3://igvf-files/2024/06/03/c11e3ed3-ed67-4621-8b10-6ef335a400f5/IGVFFI3454BUML.fastq.gz",
    "schema_version": "14",
    "seqspecs": [
        "/configuration-files/IGVFFI3154ZIRN/"
    ],
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0008566/",
        "term_name": "Illumina NextSeq 550"
    },
    "sequencing_run": 1,
    "status": "released",
    "submitted_by": {
        "@id": "/users/100f06c6-83a7-4b4d-9dfd-053a1829773b/",
        "title": "Benjamin Doughty"
    },
    "submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/221224_Cohesin_TAPseq_v2/221219_P044_cohesin_tapseq_v2/outs/fastq_path/L381_S4_L002_R1_001.fastq.gz",
    "summary": "R1 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "c11e3ed3-ed67-4621-8b10-6ef335a400f5"
}