IGVFFI3432JHMF

released

validated

File Set

IGVFDS7598WPXV(gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)

Summary

R2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jay-shendure:CRISPRa_singlecell_iPSCneuron_VPH_sequence_CS1_10Xlane4_seqlane1_R2

md5sum

4e50523fc6b83239dcd0d139ec720980

Content MD5sum

fc374547d263d23b72e2dd0965d2def9

File Size

1.5 GB

Submitted File Name

/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/neuron_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAMCTVHV/CS1_4/CS1_4_S8_L001_R2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 2000

Sequencing Run

Flowcell ID

AAAMCTVHV

Lane

Sequencing Kit

NextSeq 1000/2000 P3 Reagent Kit

Illumina Read Type

Average Read Length

Read Count

55340533

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8382KOXO

InVitroSystem

GM25256 (WTC-11)

GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4), male, Homo sapiens modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library