IGVFFI3345DMYE

released

validated

File Set

IGVFDS5620SSUW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinC-PCR4-Rep1_S63

md5sum

2b7632bc72ea744082701f3c0033dff6

Content MD5sum

15482c1ada610631083b9f67c99e2d2f

File Size

28 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinC-PCR4-Rep1_S63_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ACCAACTG:GCCA

Average Read Length

Read Count

1276054

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7889BDAF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library