IGVFFI3322NUTZ

released

validated

File Set

IGVFDS1422HBIQ(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin6hrs-FF3-BinC-PCR4_S177

md5sum

4148eec4bc61e49f1b961f5931802381

Content MD5sum

e0d789e23c57af4a756270723a12e59c

File Size

20 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin6hrs-FF3-BinC-PCR4_S177_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ACTGTATC:ATTG

Average Read Length

Read Count

850595

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2991GHKL

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library