IGVFFI3317MYOR

released

validated

File Set

IGVFDS7316VKFT(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinB-PCR1_S38

md5sum

7c25acf5763cad6906681024dd05f12c

Content MD5sum

fb4d2f3545b20dacd3413df72e3341cd

File Size

6.9 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinB-PCR1_S38_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAGCAAGG:CTCT

Average Read Length

Read Count

285261

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM8103DFGV

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library