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IGVFFI3317IHKV

released
validated
File Set
IGVFDS0334XGBU(gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M)
Summary
R1 reads from sequencing run 3
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-measurement-set-crispri-b2m-high-bin-rep3-R1
md5sum
641f86565ce5e0f84797b13d50a4a1d1
Content MD5sum
132cc88338775865c0f7344ca0eacc1b
File Size
21 MB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/B2M-High-R3_S11_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina MiSeq
Sequencing Run
3
Flowcell ID
000000000-J9HPF
Lane
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
22
Read Count
852614

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
alignments
custom assembly unfiltered alignments
Charles Gersbach, Duke
14 MB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke