IGVFFI3275ZEBW

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1937JPZI(gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW287B_1_R2
md5sum
c3d9a7062d51ca16b619ecb920548c4d
Content MD5sum
c6dbf301379d388fcec119216846e76f
File Size
1.3 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-11-03-McDermott-NextSeq.10X.run2.10X/2023-11-03/outs/fastq_path/AAF3JCVM5//LW287B_S2_L001_R2_001.fastq.gz
Checkfiles Version
Attribution

Sequencing Details

Sequencing Platform
Sequencing Run
1
Flowcell ID
AAF3JCVM5
Lane
1
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
R2
Average Read Length
90
Read Count
59476133

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW287) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
589 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
429 MB
validated