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IGVFFI3229RCYO

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS4121IORO(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW422_7_I2
md5sum
6b35953917f8208f683596d2297d1d96
Content MD5sum
582996800a8a01e890f6cc4cc534b9c2
File Size
1.9 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/scRNA//LW422_S47_L007_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NovaSeq X Plus
Sequencing Run
1
Flowcell ID
227C7WLT4
Lane
7
Sequencing Kit
NovaSeq X Series 25B Reagent Kit
Illumina Read Type
I2
Average Read Length
10
Read Count
314203724

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW422), female, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
1.9 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
513 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT