IGVFFI3191MDQA

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS0414OFOP(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinD-PCRRep1_I2

md5sum

2b96adc960209c4766aaa204e6fd36c8

Content MD5sum

fcae8386e669ce961239b7187a8a61e7

File Size

1.5 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-PCRRep1-BinD_S4_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

GAAGAAGT:TTGT

Average Read Length

Read Count

188660

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9103RFFD

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library