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IGVFFI3167FDOQ

released
validated
File Set
IGVFDS2267JZFZ(gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
md5sum
f6023820fae9b8898196616c63b2ce3a
Content MD5sum
4f8ff3a1230f126a555d0f0f8f0cc07d
File Size
7.8 MB
Submitted File Name
/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep2-FFrep1-BinD-PCRrep1_S132_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina MiSeq
Sequencing Run
1
Sequencing Kit
MiSeq Reagent Kit v2
Illumina Read Type
R1
Average Read Length
25
Read Count
338252

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin D for 76-100% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford