IGVFFI3082ZUDE

released

validated

File Set

IGVFDS9073AJJA(gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes)

Summary

R1 reads from sequencing run 2

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispra-ccr7-high-bin-rep2-R1

md5sum

ecd523d7bee18ab926ae307a8799fadb

Content MD5sum

7a70e99764479e1b6dc6a4ae7502c2c6

File Size

62 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/CCR7pos-pSM54-TF-H2_S8_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

000000000-K4RT4

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

2649829

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7634EVUP

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)