IGVFFI3017XOWT

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3617RUHJ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCAG-THP1-BioRep1-FFrep1-BinC-PCRrep1_R2
md5sum
2ed807fbaf612dc1faabcde1e6c09978
Content MD5sum
03da5eff4063fbbf39e5ee5ae9bc527b
File Size
23 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C12-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinC-PCRrep1_S877_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
R2
Index
ACTGTATC:TATGAGTA
Average Read Length
151
Read Count
1257043

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution