IGVFFI2920YFXU

released

validated

File Set

IGVFDS1862OQFF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR3_S123

md5sum

4564919a7b6a8bef5ca487273a3f95a6

Content MD5sum

0bd74d38359aff8a2a812429a09d9241

File Size

24 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinC-PCR3_S123_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GACAGTAA:ATTG

Average Read Length

Read Count

1018196

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0811AZWF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library