IGVFFI2908JEGF

released

validated

File Set

IGVFDS8967NSST(low FACS signal CRISPR knockout FACS screen sorted on the expression of IL7R integrating a guide (sgRNA) library targeting transcription start sites in many genes and an expression vector library of BATF3)

Summary

R1 reads from sequencing run 2

File Format

fastq

Content Type

reads

Aliases

charles-gersbach:cd8-tcells-measurement-set-crispr-ko-tfome-batf3-over-low-bin-rep2-R1

md5sum

4cee922eb21483efc2def02e3fa6e56b

Content MD5sum

c9b032526ffe05327465eb23d2abc399

File Size

643 MB

Submitted File Name

/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM74_L3_S9_L001_R1_001.fastq.gz

Checkfiles Version

Legacy version of checkfiles

Sequencing Details

Sequencing Platform

Illumina MiSeq

Sequencing Run

Flowcell ID

AACJJ7LM5

Lane

Sequencing Kit

MiSeq Reagent Kit v2

Illumina Read Type

Average Read Length

Read Count

40097828

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7158ADKU

PrimaryCell

CD8-positive, alpha-beta memory T cell

CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL7R in cells overexpressing BATF3) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with multiple libraries (MOI of 0.4)