IGVFFI2857MGHU

released

validated

File Set

IGVFDS6286FOBM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinB-PCR4-Rep1_S62

md5sum

b04c8d746153cf3110e725e857289f3a

Content MD5sum

8ef68048b2c79d50f8423656b6499bd5

File Size

22 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinB-PCR4-Rep1_S62_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TTGAGCCT:GCCA

Average Read Length

Read Count

1014590

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0430YSKQ

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library