IGVFFI2821MABS

released

validated

File Set

IGVFDS8183BLZH(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF1-BinE-PCR1-Rep1_S5

md5sum

7cbc6b9aa5c3e6dfc0d75a4d42759f92

Content MD5sum

47cc53339746af2272222bd0aa9bce1d

File Size

27 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF1-BinE-PCR1-Rep1_S5_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AGCAATTC:TTGA

Average Read Length

Read Count

1233647

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7435FSSP

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library