IGVFFI2772MPAA

released

validated

File Set

IGVFDS4662ZYNK(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF4-BinD-PCR3_S136

md5sum

55eb4b38baab60b7cc7608117491b9c6

Content MD5sum

e77b9d1519e32decd357373c4c0a7842

File Size

16 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF4-BinD-PCR3_S136_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CCAGAGCT:ATTG

Average Read Length

Read Count

668806

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1961BHPA

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin D for 55-65% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library