IGVFFI2762CXGD

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS1248KTOA(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

R2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCCA-THP1-BioRep1-FFrep2-FACS_Input-PCRrep2_R2

md5sum

a6f4ef057cd6ce191e854919ec35faaf

Content MD5sum

95c0da2b83e6e751f9d0db8e281f18e4

File Size

33 MB

Submitted File Name

240908-S029-5splice-THP1-5splicesite_C10-Amp_PPIF_5splicesite-BioRep1-FFrep2-BinAll-PCRrep2_S980_R2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

1

Illumina Read Type

R2

Index

AGGTTATC:AGGTGCGT

Average Read Length

151

Read Count

1782273

Samples

1 item

Accession

Type

Sample Terms

Summary

InVitroSystem

THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award

Principal Investigator(s)

Anshul Kundaje, Jesse Engreitz, Marlene Rabinovitch, Lars Steinmetz, Michael Bassik, Thomas Quertermous, Will Greenleaf

Contact P.I.

Lab

Jesse Engreitz, Stanford