IGVFFI2761AOAR

released

validated

File Set

IGVFDS5389PFCH(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin6hrs-FF1-BinC-PCR1_S3

md5sum

5471bea6726f973449e447c60f48c226

Content MD5sum

e5a6a5b2ba1d2c733ffa4d0ba9ead7a5

File Size

5.7 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin6hrs-FF1-BinC-PCR1_S3_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GCACATCT:ACGT

Average Read Length

Read Count

239950

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM6480FETB

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library