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IGVFFI2744VQUN

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS5173WYKU(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW268_1_I2
md5sum
be5448f9c6422336d17cbe71ddde81fb
Content MD5sum
c96f11ee88c74158fa1a8e427298c780
File Size
579 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-07-13-McDermott-NextSeq.10X.run3.transcriptome/2023-07-13/outs/fastq_path/AAC5GH5HV/LW268_S8_L001_I2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC5GH5HV
Lane
1
Illumina Read Type
I2
Average Read Length
10
Read Count
80629758

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
5.2 GB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
443 MB
validated