IGVFFI2737UPLU

released

validated

File Set

IGVFDS6282WCIA(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinE-PCR4-Rep1_S281

md5sum

7fe7df46ae5fc281a79de59e9943058d

Content MD5sum

f770a161221d32e09f462547c675b6dd

File Size

25 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinE-PCR4-Rep1_S281_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GTCCACAG:GCCA

Average Read Length

Read Count

1132275

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0671URTU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library