IGVFFI2735DIDC

released

validated

File Set

IGVFDS4834SKZS(gRNA sequencing for scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)

Summary

R1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jay-shendure:CRISPRa_singlecell_K562_VPR_sequence_CS1_10Xlane2_seqlane1_R1

md5sum

3f05737b8cc18e1aa15a6801fe842672

Content MD5sum

2e38bd5d1790fc1d8d76d67e3fbf53a6

File Size

873 MB

Submitted File Name

/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VPR_CS1_2/VPR_CS1_2_S8_L001_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NextSeq 2000

Sequencing Run

Flowcell ID

AAAHG2HHV

Lane

Sequencing Kit

NextSeq 1000/2000 P3 Reagent Kit

Illumina Read Type

Average Read Length

Read Count

41798174

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9542NUGS

InVitroSystem

K562

K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library