IGVFFI2718BNTR

released

validated

File Set

IGVFDS2726RPEW(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in 36 genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_PILOT_CCND1-Auxin0hrs-FF1-BinE-PCR4_S365

md5sum

159dd5919a830b47552820f53d9c7b6f

Content MD5sum

c8d9fce7ed45de29da082c44a1984ca2

File Size

9.1 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/210301B002-CohesinFF/fastq/CCND1-Auxin0hrs-FF1-BinE-PCR4_S365_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

ACAGTTGA:TGCG

Average Read Length

Read Count

389157

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM1617PVUY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library