IGVFFI2703JATP

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS0065BALX(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinC-PCRRep3_I2

md5sum

85a39c09428bdf9edbff9681a443855b

Content MD5sum

36754ebdc155b5bac7cf369d4faac4d7

File Size

1.7 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-PCRRep3-BinC_S71_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

CGCTATGT:TTGT

Average Read Length

Read Count

208099

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7206IJBO

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library