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IGVFFI2702FKSV

released
validated
File Set
IGVFDS1445OKMY(CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
richard-sherwood:18loci_uptake_Rep2_top40_read2
md5sum
4e1cd4c3379f60745fe672976b06bf71
Content MD5sum
fb5eef7ccbf42f61ddd1158e6159f4cc
File Size
12 MB
Submitted File Name
/net/bgm/sherwood/Tian_demult_data/nextseq_051523_Tian/fixed/18loci_uptake_Rep2_top40_R2.fastq.gz
Checkfiles Version
v17

Sequencing Details

Sequencing Platform
Element Biosciences AVITI
Sequencing Run
1
Illumina Read Type
R2
Average Read Length
60
Read Count
343366

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HepG2 cell line, male, Homo sapiens (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
guide quantifications
Richard Sherwood, Brigham and Women's Hospital
336 KB
validated

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Richard Sherwood, Brigham and Women's Hospital