IGVFFI2665CFFL

released

validated

File Set

IGVFDS1862OQFF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_KITLG-Auxin0hrs-FF3-BinC-PCR4_S129

md5sum

f78ebc6ea0d109fc8d733ca3d5fa4f27

Content MD5sum

d97910c0c77a27e3a41abfde04558083

File Size

24 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220104B013_Cohesin_KITLG/fastq_trim/Auxin0hrs-FF3-BinC-PCR4_S129_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CGTTACCA:ATTG

Average Read Length

Read Count

1019619

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM0811AZWF

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of KITLG) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library