IGVFFI2652BFQT

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS1533EFZO(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCCA-THP1-BioRep2-FFrep1-BinD-PCRrep4_I1
md5sum
e4ed2a125f6dd8034574b8960124e039
Content MD5sum
1593966f3dc39a6d9961514e61aff5a4
File Size
4.6 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C10-Amp_PPIF_5splicesite-BioRep2-FFrep1-BinD-PCRrep4_S818_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I1
Index
TTAATCAG:CGCAGACG
Average Read Length
8
Read Count
585184

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution