IGVFFI2646XUWX

released

validated

File Set

IGVFDS8736DPTS(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_CCND1_Auxin0hrs-FF2-BinC-PCR3-Rep1_S273

md5sum

bb0facff795c476f43cf00189b625232

Content MD5sum

aff0e8b9914dfcfec09993c3b6227819

File Size

27 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/CCND1-Auxin0hrs-FF2-BinC-PCR3-Rep1_S273_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

GCCTAGCC:GCCA

Average Read Length

Read Count

1232144

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM2887QEDU

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of CCND1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library