{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI2601VFSM/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI2601VFSM",
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "f1615ccd8f5737efe671d77320630b40",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-16T21:43:20.249137+00:00",
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS7359EZSO/",
"accession": "IGVFDS7359EZSO",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM6512KVQX/",
"accession": "IGVFSM6512KVQX",
"classifications": [
"cell line"
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"term_name": "TeloHAEC"
}
],
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin A for 0-25% expression of NOS3) modified with a guide library",
"taxa": "Homo sapiens"
}
],
"summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3"
},
"file_size": 30024270,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI2601VFSM/@@download/IGVFFI2601VFSM.fastq.gz",
"illumina_read_type": "I1",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 8,
"md5sum": "f82bf9eb206faed56c7d8131dabbfd72",
"mean_read_length": 8,
"minimum_read_length": 8,
"read_count": 3536203,
"release_timestamp": "2024-06-22T00:26:47.803553+00:00",
"s3_uri": "s3://igvf-files/2024/04/16/2842291a-9485-4e31-9ce1-7a27a9cc51c4/IGVFFI2601VFSM.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "MiSeq Reagent Kit v2",
"sequencing_platform": "/platform-terms/EFO_0004205/",
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep2-BinA-PCRrep1_S121_I1_001.fastq.gz",
"summary": "I1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "2842291a-9485-4e31-9ce1-7a27a9cc51c4"
}