IGVFFI2586BMWL

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS6025ASSH(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I1 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-BinA-PCRRep4_I1

md5sum

451a186639517f68b7b326ba5a7c405b

Content MD5sum

df09ad436c5f83acbbc885a49cc643e5

File Size

1.8 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep2-FFRep3-PCRRep4-BinA_S93_I1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

TTGTCTAT:TTGT

Average Read Length

Read Count

211886

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5388SXBN

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library