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IGVFFI2527DBCS

released
validated
File Set
IGVFDS0621TDYK(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep4-BinC-PCRrep6_R2
md5sum
b9a9337e43a3b1aab00c8cf8e906b134
Content MD5sum
555ccf45ed1e270aa33be33b14a08631
File Size
4.0 MB
Submitted File Name
230531S019-THP1-P151-Amp_PPIF_enhancer-BioRep1-FFrep4-BinC-PCRrep6_S84_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
GCCGCAAC:CAGT
Average Read Length
150
Read Count
99137

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford