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IGVFFI2509JMXD

released
validated
File Set
IGVFDS6624WHBQ(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep1-BinD-PCRrep1_R1
md5sum
1fbc00caa8f1cdeb54f014b2fdecdbc4
Content MD5sum
40938252e643ae9006564f5973b66685
File Size
8.3 MB
Submitted File Name
220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep1-BinD-PCRrep1_S25_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
2
Illumina Read Type
R1
Index
GCACATCT:ACTG
Average Read Length
150
Read Count
361474

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford