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IGVFFI2506CIAV

released
validated
File Set
IGVFDS3945FAEL(gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes)
Summary
R1 reads from sequencing run 3
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-perturb-seq-crispra-grna-rep3-rep3-fastq-lane3-R1
md5sum
95a8b1d13c188e083fe8a0e31d613e60
Content MD5sum
4ad48a09d83056f91f20293ae582ecb6
File Size
1.3 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/gRNA_CRISPRa_D3_S12_L003_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
3
Flowcell ID
HMCCWDSX3
Lane
3
Sequencing Kit
NovaSeq 6000 S4 Reagent Kit v1.5
Illumina Read Type
R1
Average Read Length
26
Read Count
65258100

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tar
filtered feature barcode matrix
gene by gene expression by CRISPR guide capture in filtered feature barcode matrix
Charles Gersbach, Duke
336 MB
validated

Associated seqspec Files

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
yaml
seqspec
seqspec of IGVFFI2506CIAV, IGVFFI3652DBNT
Charles Gersbach, Duke
1.1 KB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke