{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI2505GTMO/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI2505GTMO",
"aliases": [
"gary-hon:LW289B_1_R1"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG011996/",
"component": "functional characterization"
},
"content_md5sum": "0c1faad9b4a85698ef109246a2ebf818",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-03-04T20:06:33.667988+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS1931QVXR/",
"accession": "IGVFDS1931QVXR",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM1597KVRS/",
"accession": "IGVFSM1597KVRS",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
"status": "preview",
"summary": "CRISPRi Sp-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
"status": "preview",
"summary": "GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW289), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
"file_size": 935770104,
"flowcell_id": "AAF3JCVM5",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI2505GTMO/@@download/IGVFFI2505GTMO.fastq.gz",
"illumina_read_type": "R1",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/gary-hon/",
"title": "Gary Hon, UT"
},
"lane": 1,
"loci_list_for": [],
"maximum_read_length": 28,
"md5sum": "70a04a17519d402c15d7468b2eac0db2",
"mean_read_length": 28,
"minimum_read_length": 28,
"read_count": 44712228,
"s3_uri": "s3://igvf-files/2024/03/04/17eec6b0-32af-45d7-9027-6da89f2b8d43/IGVFFI2505GTMO.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0010963/",
"status": "released",
"term_name": "Illumina NextSeq 2000"
},
"sequencing_run": 1,
"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-11-03-McDermott-NextSeq.10X.run2.10X/2023-11-03/outs/fastq_path/AAF3JCVM5//LW289B_S4_L001_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "17eec6b0-32af-45d7-9027-6da89f2b8d43"
}