{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI2485HZST/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI2485HZST",
"aliases": [
"gary-hon:LW232_4_R1"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG011996/",
"component": "functional characterization"
},
"content_md5sum": "b0e2dc2d9c712b82a8f9811fdd6d27c0",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-02-21T20:02:13.417239+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/measurement-sets/IGVFDS9005AVSB/",
"accession": "IGVFDS9005AVSB",
"file_set_type": "experimental data",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM4953FLXN/",
"accession": "IGVFSM4953FLXN",
"classifications": [
"differentiated cell specimen"
],
"modifications": [
{
"@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
"modality": "interference",
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"summary": "CRISPRi Sp-dCas9"
}
],
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{
"@id": "/sample-terms/EFO_0009747/",
"status": "released",
"term_name": "GM25256 (WTC-11)"
}
],
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"summary": "GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW262), male, Homo sapiens modified with CRISPRi Sp-dCas9, transfected with a guide library",
"targeted_sample_term": {
"@id": "/sample-terms/CL_0000746/",
"status": "released",
"term_name": "cardiac muscle cell"
},
"taxa": "Homo sapiens"
}
],
"status": "preview",
"summary": "CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
},
"file_size": 20006501072,
"flowcell_id": "HMVTJDSX5",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI2485HZST/@@download/IGVFFI2485HZST.fastq.gz",
"illumina_read_type": "R1",
"input_file_for": [],
"integrated_in": [],
"lab": {
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"title": "Gary Hon, UT"
},
"lane": 4,
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"maximum_read_length": 151,
"md5sum": "41bdb76bf8ba16ec5376409c0d20f018",
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"s3_uri": "s3://igvf-files/2024/02/21/7ed4e72b-9bb6-48f4-9f6c-19adbe0dbfc8/IGVFFI2485HZST.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008637/",
"status": "released",
"term_name": "Illumina NovaSeq 6000"
},
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"status": "preview",
"submitted_by": {
"@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
"title": "Mpathi Nzima"
},
"submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-03-22-McDermott-NovaSeq.10X/10X/LW232_S4_L004_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "7ed4e72b-9bb6-48f4-9f6c-19adbe0dbfc8"
}