{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI2340RLRP/",
"@type": [
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"File",
"Item"
],
"accession": "IGVFFI2340RLRP",
"aliases": [
"jesse-engreitz:auxiliary_files_CRUDO_TAPseq-Auxin0hrs-Rep1_S2_R2"
],
"assay_titles": [
"TAP-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "df6d566927839494a7d20825f51a691a",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-06-03T19:14:49.065363+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS0282GEHF/",
"accession": "IGVFDS0282GEHF",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM5799ZEJH/",
"accession": "IGVFSM5799ZEJH",
"classifications": [
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],
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{
"@id": "/crispr-modifications/8543fa06-1720-4e7d-8e8f-7e24c1b1cf79/",
"modality": "interference",
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"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
},
{
"@id": "/degron-modifications/2e8d10cc-fc98-44d5-a7d9-e2892e16c98a/",
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}
],
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{
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"term_name": "HCT116"
}
],
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"summary": "HCT116 cell line, male, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
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"summary": "gRNA sequencing for mixed CRISPR TAP-seq integrating a guide (sgRNA) library targeting accessible genome regions in 29 genomic loci"
},
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"illumina_read_type": "R2",
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"/tabular-files/IGVFFI4629HCQH/"
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"integrated_in": [],
"lab": {
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"title": "Jesse Engreitz, Stanford"
},
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"maximum_read_length": 90,
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"read_count": 6480513,
"release_timestamp": "2024-07-10T20:26:34.577745+00:00",
"s3_uri": "s3://igvf-files/2024/06/03/81fed5eb-ee18-4b52-a95b-e886022e4cb6/IGVFFI2340RLRP.fastq.gz",
"schema_version": "14",
"seqspecs": [
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],
"sequencing_platform": {
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"status": "released",
"term_name": "Illumina NextSeq 550"
},
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"status": "released",
"submitted_by": {
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"title": "Benjamin Doughty"
},
"submitted_file_name": "/oak/stanford/groups/engreitz/Projects/Cohesin/221125_Cohesin_TAPseq_v1/221125_Cohesin_TAPseq_v1/outs/fastq_path/L361_S2_L002_R2_001.fastq.gz",
"summary": "R2 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "81fed5eb-ee18-4b52-a95b-e886022e4cb6"
}