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IGVFFI2320SVSF

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3671LLGR(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:240908-S029-PPIF_5SpliceSite-IndividualVariantExp-AGGT_TCCA-THP1-BioRep1-FFrep1-BinA-PCRrep2_I1
md5sum
cabc5fd39a1e65ebd41a267e9ee67bec
Content MD5sum
48477c143e31d7056c68eda149f6e1af
File Size
3.2 MB
Submitted File Name
240908-S029-5splice-THP1-5splicesite_C10-Amp_PPIF_5splicesite-BioRep1-FFrep1-BinA-PCRrep2_S783_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NovaSeq X
Sequencing Run
1
Illumina Read Type
I1
Index
CATGCTTA:CGCAGACG
Average Read Length
8
Read Count
405123

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford