IGVFFI2319YGLG

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI2319YGLG/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI2319YGLG",
    "aliases": [
        "gary-hon:LW422B_6_R2"
    ],
    "assay_titles": [
        "Perturb-seq"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG011996/",
        "component": "functional characterization"
    },
    "content_md5sum": "14bf760afbf32d5dd236263363592ce7",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-11-13T21:42:54.966414+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/auxiliary-sets/IGVFDS0170TMBA/",
        "accession": "IGVFDS0170TMBA",
        "file_set_type": "gRNA sequencing",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM8986CPUS/",
                "accession": "IGVFSM8986CPUS",
                "classifications": [
                    "differentiated cell specimen"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/0a63b754-eca4-44aa-bbc2-fccdc6c44fb5/",
                        "modality": "interference",
                        "status": "preview",
                        "summary": "CRISPRi Sp-dCas9"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0003045/",
                        "status": "released",
                        "term_name": "H9"
                    }
                ],
                "status": "preview",
                "summary": "H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW422), female, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library",
                "targeted_sample_term": {
                    "@id": "/sample-terms/CL_0000746/",
                    "status": "released",
                    "term_name": "cardiac muscle cell"
                },
                "taxa": "Homo sapiens"
            }
        ],
        "status": "preview",
        "summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci"
    },
    "file_size": 9246627465,
    "flowcell_id": "227C7WLT4",
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI2319YGLG/@@download/IGVFFI2319YGLG.fastq.gz",
    "illumina_read_type": "R2",
    "input_file_for": [],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/gary-hon/",
        "title": "Gary Hon, UT"
    },
    "lane": 6,
    "loci_list_for": [],
    "maximum_read_length": 151,
    "md5sum": "063ba3cf4bc6e8dc01dc35950fdc34d9",
    "mean_read_length": 151,
    "minimum_read_length": 151,
    "read_count": 167643252,
    "s3_uri": "s3://igvf-files/2024/11/13/056876c5-5926-4eae-acda-2fd62e73f17e/IGVFFI2319YGLG.fastq.gz",
    "schema_version": "14",
    "seqspecs": [],
    "sequencing_kit": "NovaSeq X Series 25B Reagent Kit",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0008637/",
        "status": "released",
        "term_name": "Illumina NovaSeq 6000"
    },
    "sequencing_run": 1,
    "status": "preview",
    "submitted_by": {
        "@id": "/users/b05645f1-2d56-4a5e-b272-4601e555a3fa/",
        "title": "Mpathi Nzima"
    },
    "submitted_file_name": "/project/GCRB/Hon_lab/shared/data/sequencing_data/2024/2024-05-01-Novogene-NovaSeq.Hon19_20/sgRNA//LW422B_S2_L006_R2_001.fastq.gz",
    "summary": "R2 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "056876c5-5926-4eae-acda-2fd62e73f17e"
}