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IGVFFI2309FCAH

released
validated
File Set
IGVFDS4752FLON(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230423pPPIFEnformer-Jurkat-Amp_PPIF_promoter-BioRep1-FFrep3-BinB-PCRrep1_R2
md5sum
5ae7f9ddfc558b27f25fa81adee24912
Content MD5sum
d22f72dab0b2e610fc2d6b495b1c186d
File Size
13 MB
Submitted File Name
230423pPPIFEnformerJurkat-Jurkat-PE-pPPIF_Enformer-Amp-pPPIF-BioRep1-FFRep3_BinB-PCRrep1_S109_R2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R2
Index
CATGCTTA:GTCA
Average Read Length
150
Read Count
300367

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford