IGVFFI2289LZDO

released

validated

File Set

IGVFDS9728LNRU(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin6hrs-FF1-BinA-PCR3_Rep1_S123

md5sum

93a49ecd6455ce581b9c2a3bbc7914d8

Content MD5sum

4897b7ba420487aee9da805dddfd1827

File Size

23 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin6hrs-FF1-BinA-PCR3-Rep1_S123_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

AACTTGAC:TTGA

Average Read Length

Read Count

1171429

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM3056RYCY

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin A for 0-10% expression of MYC) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library