IGVFFI2283WTXS

{
    "@context": "/terms/",
    "@id": "/sequence-files/IGVFFI2283WTXS/",
    "@type": [
        "SequenceFile",
        "File",
        "Item"
    ],
    "accession": "IGVFFI2283WTXS",
    "aliases": [
        "daniel-bauer:ChrX_Screen_ABE8e_day7_rep3_read2"
    ],
    "assay_titles": [
        "Proliferation CRISPR screen"
    ],
    "audit": {},
    "award": {
        "@id": "/awards/HG012010/",
        "component": "functional characterization"
    },
    "content_md5sum": "7e438405caa74d9f320b5b25cdb663c5",
    "content_type": "reads",
    "controlled_access": false,
    "creation_timestamp": "2024-09-10T17:37:48.934220+00:00",
    "externally_hosted": false,
    "file_format": "fastq",
    "file_set": {
        "@id": "/measurement-sets/IGVFDS7500YTSB/",
        "accession": "IGVFDS7500YTSB",
        "file_set_type": "experimental data",
        "samples": [
            {
                "@id": "/in-vitro-systems/IGVFSM9404QDZA/",
                "accession": "IGVFSM9404QDZA",
                "classifications": [
                    "cell line"
                ],
                "modifications": [
                    {
                        "@id": "/crispr-modifications/df6a701e-a9f4-4908-a5fd-8d6687c2694d/",
                        "modality": "base editing",
                        "status": "in progress",
                        "summary": "CRISPR base editing SpRY-ABE8e"
                    }
                ],
                "sample_terms": [
                    {
                        "@id": "/sample-terms/EFO_0022838/",
                        "status": "released",
                        "term_name": "HUDEP-2"
                    }
                ],
                "status": "released",
                "summary": "HUDEP-2 cell line, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library",
                "taxa": "Homo sapiens"
            }
        ],
        "status": "released",
        "summary": "Proliferation CRISPR base editing screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide"
    },
    "file_size": 125500378,
    "gene_list_for": [],
    "href": "/sequence-files/IGVFFI2283WTXS/@@download/IGVFFI2283WTXS.fastq.gz",
    "illumina_read_type": "R2",
    "index": "ACGTAgat",
    "input_file_for": [
        "/tabular-files/IGVFFI9446LEJP/"
    ],
    "integrated_in": [],
    "lab": {
        "@id": "/labs/daniel-bauer/",
        "title": "Daniel Bauer, HSCI"
    },
    "loci_list_for": [],
    "maximum_read_length": 150,
    "md5sum": "2ba28902c737106bc6fa4dfac31e4cf4",
    "mean_read_length": 150,
    "minimum_read_length": 150,
    "quality_metrics": [],
    "read_count": 2957115,
    "release_timestamp": "2025-03-07T23:04:56.954666+00:00",
    "s3_uri": "s3://igvf-files/2024/09/10/f6dcb58d-734c-47cf-a211-4830c783a2ca/IGVFFI2283WTXS.fastq.gz",
    "schema_version": "15",
    "seqspecs": [],
    "sequencing_kit": "NovaSeq X Series 10B Reagent Kit",
    "sequencing_platform": {
        "@id": "/platform-terms/EFO_0022840/",
        "status": "released",
        "term_name": "Illumina NovaSeq X"
    },
    "sequencing_run": 1,
    "status": "released",
    "submitted_by": {
        "@id": "/users/96fd4846-bfa6-47db-ac12-495df29943ee/",
        "title": "Eric Che"
    },
    "submitted_file_name": "/data/pinello/PROJECTS/2024_02_EC_ChrX/submitted_files/ChrX_Screen_1_#ACGTAgat_R2.fastq.gz",
    "summary": "R2 reads from sequencing run 1",
    "upload_status": "validated",
    "uuid": "f6dcb58d-734c-47cf-a211-4830c783a2ca"
}