IGVFFI2265SRGR

released

validated

File Set

IGVFDS8794YVNF(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF1-BinF-PCR4_Rep1_S24

md5sum

20221255548487ba8ac390f9bdf3bb3a

Content MD5sum

0b74bffdd2e63632610fff22830cfb21

File Size

42 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF1-BinF-PCR4-Rep1_S24_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TACTTAGC:TGCG

Average Read Length

Read Count

2187211

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM5744WHTT

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin F for 80-100% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library