IGVFFI2184WUSE

released

validated

File Set

IGVFDS6371DGFM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin0hrs-FF2-BinB-PCR2-Rep1_S20

md5sum

4e7653365f8b99ec2aec3995f80b89bc

Content MD5sum

0e2037813b215cb9113860e1caa70b11

File Size

9.3 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin0hrs-FF2-BinB-PCR2-Rep1_S20_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CAGGAGCC:TTGA

Average Read Length

Read Count

424189

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9860IGWK

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin B for 10-20% expression of SSFA2) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library