IGVFFI2183ONZB

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS2408JJSR(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-BinB-PCRRep1_I2
md5sum
aedcac6a2a30ff4a68f29465d41a13d9
Content MD5sum
3f736bacaa771bd16308d4af995abd29
File Size
1.4 MB
Submitted File Name
Jurkat_Stim-IL2RA_prom-BioRep1-FFRep2-PCRRep1-BinB_S6_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Sequencing Run
1
Illumina Read Type
I2
Index
TCCTTGGT:TTGT
Average Read Length
4
Read Count
177822

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
tsv
variant effects
variant effects
Jesse Engreitz, Stanford
5.9 KB
validated

Attribution