{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI2078FUDW/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI2078FUDW",
"aliases": [
"charles-gersbach:cd8-tcells-perturb-seq-crispri-grna-rep2-rep2-fastq-lane1-R1"
],
"assay_titles": [
"Perturb-seq"
],
"audit": {},
"award": {
"@id": "/awards/HG012053/",
"component": "functional characterization"
},
"content_md5sum": "980f4f71889bb43e2f7c63422d41e04d",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2023-11-09T19:34:19.024803+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS2399UCGA/",
"accession": "IGVFDS2399UCGA",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/primary-cells/IGVFSM2747RJFY/",
"accession": "IGVFSM2747RJFY",
"classifications": [
"primary cell"
],
"modifications": [
{
"@id": "/crispr-modifications/95393c9a-637d-43f6-baf9-662e45d28d61/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sa-dCas9"
}
],
"sample_terms": [
{
"@id": "/sample-terms/CL_0000909/",
"status": "released",
"term_name": "CD8-positive, alpha-beta memory T cell"
}
],
"status": "released",
"summary": "CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes"
},
"file_size": 2210702257,
"flowcell_id": "HMCCWDSX3",
"gene_list_for": [],
"href": "/sequence-files/IGVFFI2078FUDW/@@download/IGVFFI2078FUDW.fastq.gz",
"illumina_read_type": "R1",
"input_file_for": [
"/matrix-files/IGVFFI0547WZWK/"
],
"integrated_in": [],
"lab": {
"@id": "/labs/charles-gersbach/",
"title": "Charles Gersbach, Duke"
},
"lane": 1,
"loci_list_for": [],
"maximum_read_length": 26,
"md5sum": "1643cc4319236504963ac71399311647",
"mean_read_length": 26,
"minimum_read_length": 26,
"read_count": 108176366,
"release_timestamp": "2024-10-18T19:50:07.190077+00:00",
"s3_uri": "s3://igvf-files/2023/11/09/19b9654a-4da7-4d1e-afc9-28cc340ce17c/IGVFFI2078FUDW.fastq.gz",
"schema_version": "14",
"seqspecs": [
"/configuration-files/IGVFFI0543IRBP/"
],
"sequencing_kit": "NovaSeq 6000 S4 Reagent Kit v1.5",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0008637/",
"status": "released",
"term_name": "Illumina NovaSeq 6000"
},
"sequencing_run": 2,
"status": "released",
"submitted_by": {
"@id": "/users/29f5f78d-ac90-421e-90c6-a90e1dc407a7/",
"title": "Alejandro Barrera"
},
"submitted_file_name": "/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/gRNA_CRISPRi_D2_S8_L001_R1_001.fastq.gz",
"summary": "R1 reads from sequencing run 2",
"upload_status": "validated",
"uuid": "19b9654a-4da7-4d1e-afc9-28cc340ce17c"
}